Petri Dish Platinum Electrode

Product Introduction and Description

This product is a package that includes an electrode and a platinum petri dish. The system consists of a glass petri dish equipped with a platinum electrode and a rod with a platinum electrode that is placed on top of the tissue. By placing the tissue sample between these two square electrodes, a uniform electric field is created across the tissue, enabling effective molecule transfer.

The platinum petri dish electrodes and chambers for tissues are an advanced tool for ex-vivo tissue electroporation. This product is specifically designed for gene or drug delivery to in-vitro tissue samples and allows for the effective transfection of even large or unusually shaped tissues that are difficult to transfect with standard electrodes. As a result, this system provides an efficient method for delivering genes, drugs, and other molecules to various tissues (such as cornea, muscle, skin, etc.) under in-vitro conditions.

In this device, controlled electrical pulses or electroporation technology is used to temporarily increase the permeability of the cell membranes in the tissue. This allows large molecules such as plasmid DNA, RNA, proteins, or various drugs to be directly introduced into the tissue cells. This feature makes electroporation a powerful tool in biomedical research, serving as an effective alternative to viral gene transfer methods or invasive direct injection techniques.

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Product Applications in Gene and Drug Delivery to Ex-Vivo Tissues

The platinum petri dish electrode has a wide range of applications in various scientific fields, particularly in gene delivery and drug delivery to ex-vivo tissues using electroporation. These applications include:

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• Tissue Engineering and Regenerative Medicine: In tissue engineering, it is often necessary to deliver specific genes or growth factors to cells within cultured tissues to promote proper differentiation or tissue repair. This product enables efficient gene transfer to tissue scaffolds or cultured organs under laboratory conditions.
• In-Vitro Gene Therapy Research: Many gene therapy studies require gene transfer to diseased tissues (e.g., tumor tissues removed from patients) in a laboratory setting. Using this electroporation chamber, therapeutic genes or genome editing tools like CRISPR/Cas9 can be uniformly introduced into the target tissue, which can then be prepared for re-implantation into the patient or for further studies.
• Drug Delivery to Living Tissues: In pharmacological studies aimed at examining the effects of drugs on living tissues, this device can be used to electrically inject drug molecules or nanoparticles directly into the tissue. This method is particularly useful for drugs with low tissue penetration, helping to increase local drug concentration within the tissue.
• Specialized Tissue Samples (Neuroscience and Others): In neuroscience, ex-vivo brain slices are used to study neural networks and neuronal growth. The platinum petri dish electrode allows for the high-efficiency introduction of reporter genes or neuronal stimulators into thin brain slices. Similarly, in other sensitive tissues such as the cornea or skin, this tool enables effective and rapid molecular delivery.

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Advantages and Unique Features

This product has outstanding features that distinguish it from traditional methods and tools:

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• Special Design for Large or Unusual Tissues: The petri dish chamber features square platinum electrodes optimized for securely holding larger or irregularly shaped tissues. This design addresses the challenge of transfecting difficult-to-penetrate tissue samples and allows for experimentation on various types of tissue samples.
• Uniform Electric Field and High Efficiency: The arrangement of two opposing electrodes (one at the bottom of the dish and the other at the end of the rod) creates a homogeneous electric field throughout the tissue. This uniformity ensures that all parts of the tissue are equally exposed to electroporation, optimizing molecule uptake (transfection).
• Platinum Electrodes in a Neutral Silicone Base: The electrodes in this system are made of pure platinum embedded in a neutral silicone base. Platinum is a highly stable and non-reactive conductor, ensuring no corrosion or chemical reactions that could damage the sample during electrical pulsing. The silicone base provides soft, neutral support for the tissue, maintaining its shape during the process.
• Pyrex Glass Petri Dish (Sterilizable): The main chamber is made of laboratory-grade Pyrex glass, which has high thermal and chemical resistance and can be sterilized for reuse (e.g., via autoclaving or chemical disinfection). The reusability of this dish reduces laboratory costs and is environmentally friendly.
• Two Sizes for Sample Adaptation: This product is available in two models with different distances between the electrodes. Thesmaller model is suitable for thinner tissues or small pieces, while the larger model accommodates thicker or larger tissues. Researchers can choose the appropriate option based on the size of their sample to achieve the best results.
• Easy Setup and Use: The kit is designed for straightforward use in the laboratory. Simply place the tissue in the chamber, position the top electrode (attached to the rod) on the tissue, and connect the cables to the electroporation device to prepare the system for pulsing. This simplicity allows researchers to proceed with their experiments without wasting time.
• Compatibility with Common Electroporation Devices: The platinum petri dish electrode is fully compatible with electroporation devices, including those from Pars Tarava. The included micrograbber cables facilitate easy connection to various electroporation generator outputs.

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Technical Information and Essential Details for Researchers

To make the most of the platinum petri dish electrodes and chambers for tissues, it is helpful to be aware of the following technical specifications and practical tips:

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• Voltage Range and Pulse Duration: This system can apply electrical pulses with voltages up to 200 V. The pulse duration can be adjusted from 10 microseconds to 100 milliseconds. This wide range allows researchers to find and apply the optimal conditions for the penetration of the desired molecules into the tissue.
• Recommended Pulse Waveform: Given the electrode design, square wave pulses are recommended for this system, as they are highly effective in tissue electroporation. Square waves help create uniform temporary pores in the membrane, facilitating molecule entry.
• Sample Capacity and Chamber Dimensions: The square electrode inside the petri dish provides sufficient space for placing a tissue piece. Typically, a standard 100 mm petri dish is used in this kit. The square chamber at the bottom of the petri dish holds the tissue in place and ensures proper electrical contact.
• Kit Components: The complete package includes a glass petri dish, a lid or top electrode rod, and connecting cables (micrograbbers). Having all components in one package eliminates the need for additional equipment and speeds up the experiment setup process.
• Safety and Biocompatibility: The materials used (Pyrex glass, platinum, neutral silicone) are all biocompatible and non-toxic. Platinum’s high corrosion resistance ensures no harmful metal ions are released into the environment, and the neutral silicone does not react with the culture medium or tissue sample. These features guarantee that the observed responses in the tissue are solely due to the electrical pulse and introduced molecules, without interference from external factors.
• Application in Delicate Tissues: For highly sensitive or thin tissues (such as brain slices or epithelial tissues), this system operates more gently than other methods. The soft silicone base supports the tissue, and the top rod is designed to apply minimal physical pressure to the sample. Therefore, mechanical damage to the tissue during electroporation is minimized.
• Successful Research Examples: Numerous researchers have successfully used this tool for various purposes. For instance, in neurodevelopmental studies, gene transfer to mouse brain slices has been successfully achieved using this system. Additionally, reports indicate that in delivering genes to ex-vivo skin and muscle tissues, the platinum petri dish electrode has resulted in high efficiency and uniform gene expression. Such results demonstrate the high efficiency and reliability of this product in scientific research.

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Summary

Considering all these features and technical information, the platinum petri dish electrodes and chambers for tissues are an ideal choice for researchers seeking a reliable, efficient, and flexible method for transferring genes and molecules to ex-vivo tissues. This product, with its intelligent design, facilitates complex experiments in tissue engineering, cell and molecular biology, gene therapy, and other related fields. It also provides reliable results that can contribute to advancing the frontiers of knowledge in biomedical sciences.

The product has successfully passed all necessary tests for standardization in accredited medical equipment laboratories and is ready for use in your research. For consultation, to receive the catalog, or to make a purchase, you can contact us at +989024051862 or via WhatsApp.

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