Square Electrodes for Electroporation

The product includes the following:

– A square-shaped electrode mounted at the tip of a holder rod
– A square-shaped electrode mounted on a Petri dish

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Product description and Components

This package contains two square-shaped electrodes specifically designed for conducting electroporation processes in laboratory environments. The first electrode is a square plate mounted at the bottom of a Petri dish, and the second is also square-shaped with similar dimensions, positioned at the end of a holder rod (tip of stick). Both electrodes are made from a core conductive metal coated with pure platinum, which ensures high conductivity, chemical stability, and excellent biocompatibility. These electrodes are fully compatible with electroporation devices manufactured by Pars Tarava (the first electroporation device manufacturer in Iran) and feature standard electrical connectors to the device’s output.

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Applications and Uses

The square electrodes designed for this product can be used in a wide range of biomedical research applications. The most prominent application is the transfer of large molecules, such as DNA, RNA, proteins, or drugs, into cells and tissues via electroporation.

This tool is particularly useful in the fields of tissue engineering and cell biology. Applications include the transfer of plasmids containing modified genes into mammalian cells to produce laboratory mouse models, gene therapy using cultured cells, or the introduction of drug molecules into three-dimensional tissue cultures. These electrodes can also be used for research on tissue samples such as hippocampal tissue slices from mice or avian embryos, where placing the tissue slice between the two electrodes allows for uniform electric field penetration. The areas where electroporation technology can be applied are summarized as follows:

• Gene and RNA Transfer Research: Cell culture tests and the production of transgenic organisms and disease models.
• Tissue Engineering and Cell Biology: Culturing and differentiating stem cells under the influence of foreign genes or growth factors.
• Multicellular Tissue Studies: Electroporating tissue slices and early embryos (such as chicken embryos) to study development or diseases.
• Biotechnology and Pharmacology: Targeted delivery of therapeutic proteins, antibiotics, or cellular vaccines into living cells.

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Advantages of Square-Shaped Design

The square design of the electrodes ensures a more uniform distribution of the electric field in the area between the two electrodes, so that the cells and tissues between the electrodes are exposed to a similar field intensity. Studies have shown that flat (plate) electrodes create a more uniform field compared to sharp-pointed electrodes.

The flat and wide surface of the square electrode also provides a larger coverage area compared to circular (disc-shaped) electrodes, increasing the contact area with the sample. Another benefit is the more precise control of the field direction. The square shape and its clear angles allow for better electrode alignment, ensuring a more predictable electric field direction.

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The benefits of the square electrode design are summarized as follows:

• More Uniform Electric Field Distribution: The parallel plate arrangement guarantees uniform electric field distribution across the entire tissue between the two electrodes.
• Larger Contact Area: The larger surface area of the square plate increases the efficiency of molecule transfer into cells.
• Stability and Alignment Accuracy: The symmetrical geometric design enhances alignment and improves the reproducibility of results.
• Compatibility with Larger Samples: The design is suitable for electroporating larger or adherent tissues on culture surfaces.

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Key Technical Features

• Electrode Shape: Each electrode is square-shaped, and its size is consistent with standard research electrode specifications.
• Material and Construction: Platinum is chosen for its high chemical stability and excellent electrical conductivity, making it an ideal choice for biological electrodes.
• Frame: The Petri dish electrode includes a frame to ensure the sample is positioned at the optimal distance from the electrode during pulse application, preventing direct contact between cells and the electrode.
• Device Compatibility: The electrical connectors of these electrodes are fully compatible with Pars Tarava’s electroporation devices, allowing for direct and secure connection to the device’s high-voltage outputs.
• Durability and Maintenance: The electrode surface is resistant to corrosion and can be cleaned with standard laboratory solutions. For sterilization, common laboratory sterilization methods can be used.

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Safety and Device Compatibility

Electroporation involves applying high-voltage pulses to living tissues, so adhering to safety standards is crucial. The square electrode designed for this package meets international electrochemical safety standards and ensures resistance to unwanted discharges, as well as no chemical reactions with the culture environment.

The square electrode for electroporation is designed to have low thermal conductivity, which reduces the likelihood of localized heating. Additionally, the insulated shape and precise dimensions of these electrodes ensure that the performance of the Pars Tarava device, in terms of voltage regulation and pulse duration, is fully maintained. In other words, the electrode package is designed to ensure uniform electric field distribution, which is essential for safe and effective electroporation.

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This product has passed all the necessary tests to meet the required standards in accredited medical equipment laboratories and is ready for use in your research. For consultations, catalog requests, and purchasing, you can contact us at +98 902 405 1862 via WhatsApp or email us at info@persiantarava.me.

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